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American Society of Microbiology ABMM Sample Questions:

1. A laboratory worker sustains a needlestick injury while handling a blood sample known to be positive for Hepatitis B surface antigen (HBsAg) and Hepatitis B e antigen (HBeAg). The worker has completed the HBV vaccination series and has a documented anti-HBs titer >10 mIU/mL one month ago. What is the recommended post-exposure prophylaxis?

A) Hepatitis B vaccine booster dose only
B) Both HBIG and HBV vaccine booster dose
C) Hepatitis B immune globulin (HBIG) only
D) No treatment required

2. A clinical microbiology laboratory is performing antimicrobial susceptibility testing on a Streptococcus pneumoniae isolate recovered from a patient with meningitis. The isolate shows reduced susceptibility to penicillin (MIC = 2 µg/mL). The MOST likely mechanism for this reduced susceptibility is:

A) Modification of the peptidoglycan structure, preventing penicillin binding.
B) Alterations in the penicillin-binding proteins (PBPs) that reduce their affinity for penicillin.
C) Increased activity of efflux pumps that actively remove penicillin from the cell.
D) Production of beta-lactamase enzymes that hydrolyze penicillin.

3. A microbiology laboratory receives a corneal scraping from a patient with keratitis. Microscopic examination with calcofluor white stain reveals the presence of septate hyphae and conidiophores with a foot cell and phialides. The MOST likely genus is:

A) Mucor
B) Rhizopus
C) Aspergillus
D) Candida

4. A clinical microbiology laboratory is monitoring the emergence of resistance to colistin in Gram-negative bacteria. Resistance to colistin is often mediated by modifications of the bacterial lipopolysaccharide (LPS).
The genetic basis for acquired colistin resistance most commonly involves:

A) Mutations in genes encoding enzymes that modify the lipid A component of LPS.
B) Production of enzymes that directly inactivate the colistin molecule.
C) Acquisition of genes encoding efflux pumps that specifically target colistin.
D) Alterations in the outer membrane porin proteins, reducing colistin uptake.

5. A Gram-positive rod is isolated from the blood culture of a neonate with sepsis. The organism exhibits weak beta-hemolysis on sheep blood agar, is catalase-positive, hydrolyzes esculin in the presence of bile, and demonstrates characteristic "tumbling" motility in a wet mount prepared from a broth culture incubated at 25°C. Which organism is most likely?

A) Listeria monocytogenes
B) Bacillus anthracis
C) Corynebacterium jeikeium
D) Erysipelothrix rhusiopathiae

Solutions: